PROCESS OF RECOMBINANT DNA TECHNOLOGY

The process of recombinant DNA technology involves ;

I- Isolation of genetic material

The gene of interest is isolated from the cells by using the enzymes lysing as it dissolved the plasma membrane of the cell.

II- Cutting of the genetic material at specific site 

The desired gene or the gene of interest is then cut into specific site with the help of the enzyme the restriction endonucleases .These enzymes cut the DNA segment forming sticky ends .

III- Formation of recombinant DNA 

Then the gene of interest is inserted into the plasmid vector with the help of the enzyme DNA ligase . After insertion they are called as the recombinant DNA .

IV- Transformation of the recombinant DNA into the competent host 

The recombinant DNA is then transformed into the host or competent  host cells . 

Here the plasmid DNA replicates independently along with the genetic material of the host .

This results in the formation of multiple copies of the desired DNA or gene .