The fragments of DNA are separated by a technique called gel electrophoresis .
The DNA is cut into fragments by restriction endonucleases .
These fragments are separated by a technique called gel electrophoresis .
Agarose a natural polymer obtained from sea weeds is used as the matrix .
DNA fragments being negatively charged are separately by forcing them to move through the matrix towards the anode under an electric field .
The DNA fragments separate according to their size .
The separated molecules are stained by ethidium bromide and visualised by exposure to UV radiation as bright orange coloured bands .
The separated bands of DNA are cut from the gel and extracted from the gel piece .
Such DNA fragments are purified and used for constructing recombinant DNA by joining them with cloning vectors .
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