ENZYME FUNCTION IN REPLICATION
1- Topoisomerase - Initiation of unwinding of helix . It detangles the coils and super coils .
2 - Helicase - Breaks the hydrogen bonds between two stranded DNA becomes single stranded.
3 - Single stranded- These bind to the single strands of DNA to prevent hydrogen bonding and reforming binding proteins of duplex .
4 - DNA polymerases- DNA pol-III addition of nucleotides to the newly synthesized DNA strand DNA pol-I proof freading .
5 - Primase - Synthesis of primer .
6 - DNA ligase - Joins the DNA by forming phosphodiester bond .
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